However, white, creamy friable callus was obtained when explants were cultured on MS medium supplemented with 2, 4-D alone. Gerbera jamesonii is also used in the preparation of traditional Chinese medicine, tu-er-feng, for curing cold and also for treating rheumatism Ye et al. Micropropagation is the main system used to clonally propagate gerbera in vitro resulting in the production of millions of plantlets each year. Micropropagation of Gerbera Gerbera jamesonii Bolus.
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Brat However, in this study, at a very high concentration of BAP and other growth regulators, development and growth of shoots were inhibited. Numerous types of explants and protocols for micropropagation have been established and used for gerbera. Rooted plantlets of Gerbera were successfully established and all plantlets were transferred to soil and maintained in the green house.
Cytokinin alone in the culture medium induces shoot formation in many plants. In Mocropropagation jamesoniiBAP was also required for the formation of callus. There was a problem providing the content you requested Percentage of shoot formation and number of shoots per explants produced on MS medium supplemented with different hormones and concentrations after 8 weeks in culture supplemented with 2.
How to cite this article: However, in Gerbera jamesoniicytokinin alone in the medium induces the formation of microshoots. The combination of low concentration of cytokinin and auxin initiated propagation of Begonia species Reuter and Bhandari, It is reported that a good combination of cytokinin and auxin in the culture medium enhanced good shoot formation and plantlet regeneration from explants, for example, adventitious Gerbera shoots were regenerated from flower buds of greenhouse grown plants Pierik et al.
Because genetic variability within the Gerbera genus is relatively limited, breeding potential for new flower colours and patterns as well as resistance to biotic or abiotic stresses is also limited Orlikowska et al. Lower concentration of TDZ promoted direct shoot regeneraration and higher concentrations promoted callus induction in Hagenia abyssinica Bruce J.
However, higher addition of auxin compared to cytokinin in the culture medium resulted in the inhibition of shoot formation. Hook by tissue culture technique. However, in Gerberaaddition of charcoal is not mcropropagation for rooting.
Principles of Plant Biotechnology. Based on the present study, all combinations of hormones used were able to induce callus. Epigenetic variations in micropropagated gerbera are frequently observed only with high concentrations of cytokinins in the culture medium but somaclonal variation is rare. Micropropagation of ornamental plants-cut flowers. Direct shoot mixropropagation from lamina explants of two commercial cut flower cultivars of Anthurium andraeanum Hort. In Begoniathe addition of kinetin and zeatin in the culture medium Murashige and Gerbear, induced multiple shoots Jain, Ornamental plants and woody plant species are also propagated commercially by axillary bud proliferation Mantell et al.
However, white, creamy friable callus was obtained when explants were cultured on MS medium supplemented with 2, 4-D alone. Gerbera plantlets from in vitro cultivated capitulum explants. Survival rate of micro propagated plants that were transferred to soil were investigated Fig.
However, regeneration of root was not observed when explants were cultured on MS medium supplemented with BAP and 2, 4-D. Tissue culture technique has long been used since the cell theory was established.
The most important aspect in plant tissue culture is the capability of cultured cells and tissues to regenerate into complete plants. Shoot tips are the microopropagation used explant while adventitious shoot induction from the capitulum is also a popular method. Micrporopagation Optimization and Organogenesis of Gerbera jamesonii Bolus ex. In vitro Culture of Higher Plants. A revised medium for rapid growth and bio assays with tobacco tissue cultures.
In conclusion, the research done has proven that micro propagation of Gerbera jamesonii Bolus ex. The miceopropagation of action of thidiazuron: Organogenesis and histogenesis of adventitious organ induced on leaf blade segments of Begonia elatior hybrids Begonia x hiemalis in tissue culture. Callus is capable of forming adventitious roots.
In vitro plantlet production from young capitulum explants of Gerbera jamesonii. Springer-Verlag, New York, pp: In vitro regeneration, callus induction and root formation were optimized by manipulation of growth regulators during organogenesis. The lowest shoot formation was observed when explants were cultured on MS medium supplemented with 0.
Auxins, indoleamines and ion channels in the regeneration of Echinacea purpurea L. The propagation rates via organogenesis can be much higher than axillary shoot proliferation Chun, Adventitious organogenesis or shoot formation is a preferred system as it enables to retain the clonal fidelity since many ornamental species are cultivars that are propagated for one or more unique characteristics Kantia and Kothari, In vitro propagated Gerbera plants were compared morphologically with the intact plant.
Most papers in the literature have focused on testing the influence of different types and combinations of plant growth regulators with the aim of improving the regeneration and multiplication stage of one or few cultivars. The studing of micropropagation of Gerbera sp via shoot tip explant. The physiological status of the donor is determined by environmental conditions such as temperature, light intensity, day-length and light wavelength.
Factors affecting differentiation in vitro and mass propagation scheme for Begonia x hiemalis. Takayama and Misawa reported that medium containing 0. They are planted outdoors in full sun and useful as cut flowers, pot plant and also bedding plant.
In vitro establishment and plantlet micrropropagation of Gerbera jamesonii Bolus ex. Many microproppagation have tried to prove the totipotency concept, which is the ability of a single cell to form complete individual.
GERBERA MICROPROPAGATION PDF
Nir Micropropagation of Gerbera Gerbera jamesonii Bolus. In the present study, experiments were conducted to investigate organogenesis from various sources of Gerbera explants. This callus gave the highest dry weight. Combination of auxin and cytokinin induces the formation of adventitious shoots and roots. Plantlets survival rate achieved was Hook by tissue culture technique. Root explants were found to be non-regenerative in all experiments conducted. Effects of growth regulators and incubation period on in vitro regeneration of adventitious shoots from Gerbera petioles.
Micropropagation of Gerbera (Gerbera jamesonii Bolus).
Mezitilar However, in this study, at a very high concentration of BAP microproapgation other growth regulators, development and growth of shoots were inhibited. Gerbera jamesonii is also used in the preparation of traditional Chinese medicine, tu-er-feng, for curing cold and also for treating rheumatism Ye et al. Studies of Gerbera clonal propagation could also be efficiently adapted for other ornamental plants. The first step mivropropagation de novo regeneration is to establish callus or cell suspension cultures.